Age
and Growth Rates of Freshwater Mussels
By: James L. Anthony
Although
freshwater mussels are quite diverse in North America, they
are declining at an alarming rate. To develop the management
and recovery plans necessary for the conservation of these
animals, we need to understand their population dynamics.
This depends largely upon a knowledge of their age and growth
rates. My research involves the examination of the validity
of current aging methods for freshwater mussels in Iowa. These
methods which rely on various shell markings or banding patterns,
may substantially underestimate age and overestimate growth
rates. I am studying the validity of these techniques through
a one year mark- recovery project in streams throughout the
state of Iowa. Additionally, I am investigating various radiometric
and chemical dating methods for use with living freshwater
mussels. These methods may provide additional insight into
the true age and growth dynamics of these mysterious creatures.
Assessing
Iowa's Inland Stream Mussel Populations
By: Kelly Arbuckle
The importance of freshwater mussels has been recognized
for decades. Historically, mussels were prized for their commercial
value in the production of pearl buttons. More recently, their
economic value is attributed to the Japanese culture-pearl
industry. They are also important ecologically as a food source
for terrestrial and aquatic animals and as water quality indicators.
Once common in Midwestern rivers and streams, mussels are
now reported as more at risk than any other group of animals.
There are numerous biological, chemical, and physical factors
adversely influencing freshwater mussel communities. These
include commercial exploitation, lack of host fishes, exotic
species (e.g. zebra mussels), and habitat degradation and
destruction. Understanding the relationship between these
factors and mussel populations is important to the management
of these animals. Funded by the Iowa
Department of Natural Resources, this project focuses
on assessing inland stream mussel populations and identifying
environmental variables influencing their distribution. The
study includes a field survey to quantitatively assess current
populations and GIS analysis to describe physical factors
impacting Iowa mussels.
More
About the Project
Statement
of Purpose
Frest (1987) described mussel populations in most major
Iowa streams as declining. He suggested at least half of Iowas
interior stream mussel faunae should be considered in danger
of extirpation. Frest recommended future studies including
an examination of additional streams and more extensive surveys
of several surveyed streams. Frest's report is the most current
assessment of Iowas mussel faunae, but evaluated only
a fraction of the streams having the potential to support
mussel populations. A thorough assessment, including re-surveying
sites of previous studies, evaluating new streams, quantification
of abundance and impacts on mussel faunae in Iowa, is needed.
The purpose of this study is to provide the Iowa Department
of Natural Resources with a current assessment of Iowas
lotic mussel populations. The study will include a field survey
to assess current populations and GIS (Geographic Information
Systems) analysis to identify factors impacting mussel populations.
Description of Work and Services
Field Survey
Re-survey old sites: The study will
re-sample inland streams, as many sites as is feasible to
complete in one field season, where mussels were previously
reported by Frest (1987). The study will be completed by
a graduate research assistant, technicians, and trained
volunteers. Quantitative estimates of mussel abundance will
be made. Data collected from streams previously surveyed
will be used to assess changes in the condition of mussel
populations over time.
Survey new sites: The study will include
a survey of inland streams not previously surveyed. Recommendations
from the previous study (Frest 1987) will be followed. The
graduate assistant, technicians, and trained volunteers
will follow a protocol similar to that of Frest (1987) to
survey inland streams, as many sites as can be practically
completed in one field season, having the potential to support
freshwater mussel faunae. New baseline data will be quantitative,
following the precision protocol of Downing and Downing
(1992).
Physical factors: The study will describe
possible physical factors influencing the distribution of
inland stream mussel faunae. The study will include a description
of instream and riparian habitats where mussels are observed
or are not observed. The data collected will include canopy
closure, land use within the watershed, stream bank vegetation,
habitat type (e.g. riffle, run, pool), water quality (suspended
sediments, alkalinity, nutrients, dissolved oxygen), and
sediment characterization.
Volunteer recruitment and training: To expedite
the survey of previously identified sites and to acquire
broad data sets in a way not otherwise achievable, the study
will recruit private citizens to assist in fieldwork. Local
volunteers will be trained to use standardized methods to
collect and report data. Volunteers will provide preliminary
assessment of potential survey sites and/or work with the
research assistant or technicians on extensive stream surveys.
Volunteer training workshops: Volunteers will
attend workshops to learn standardized methods for collecting
and reporting data. Topics covered in workshops will include
how to use inventory tools (e.g. topographic maps) to accurately
locate a study site, and the collection, recording, and
reporting habitat data in and around the stream channels.
Environmental and conservation ethics will be addressed.
Create GIS coverage from existing data
sources: Using existing data (Frest 1987), the study
will digitize survey sites and create a GIS database of freshwater
mussel distribution in previously examined inland streams.
The streams formerly surveyed are located within the Paleozoic
Plateau, and the Wapsipinicon, Cedar, Iowa, and Des Moines
river systems.
Incorporating proposed study data: The re-survey
data collected and data collected from new sites will be added
to the GIS database (see GIS/Predictive Study, item #1).
Description of watershed characteristics: A
description of land uses in the watershed will be made from
the records of the county Natural Resources Conservation Service
Office, most recent aerial photography, other studies, GIS
databases (e.g. river and topography data at www.igsb.uiowa.edu/nrgis/gishome.htm)
and field surveys.
Geological description of basin: Using existing
records, the study will provide a geological description of
the drainage basin including soil types. Soil types will be
summarized from the Digital Soil Survey files in ARC/INFO
GIS export format. These files are a digital representation
of the Natural Resources Conservation Service soil survey
report. Soil mapping units in these files are attributed and
can be linked to the Iowa Soil Properties Interpretation Database
(ISPAID).
Habitat description: The study will provide
a description of habitat requirements (by species) for Iowa
freshwater mussels. Variables that relate to species habitat
requirements will be identified and associated with existing
GIS data bases.
Descriptive and predictive analysis: The study
will use descriptive analysis to correlate watershed characteristics
with presence, absence, and abundance of mussels. The study
will develop and apply GIS predictive analysis and multiple
regression analysis to help locate other inland stream sites
supporting freshwater mussel faunae using the GIS coverages
created in this study (see item #1) and existing GIS coverages
(identified above as related to species habitat requirements).
The predictive analysis can be used to guide future field
surveys.

Mussel
Trivia
| For
some freshwater mussel species, a host is necessary
for its larvae (glochidia) to develop and disperse.
Most of the time, the host animal is a fish. As such,
mussels have some amazing strategies to attract the
appropriate host. Some mussels go fishing by waving
a minnow-like lure (a modification of the mantle) in
the stream current. If successful, the glochidia attach
themselves to the gills or fins of a fish attracted
to the lure. The fish carries the mussels for 3 weeks
to several months depending on the species. Eventually,
juvenile clams fall from the fish to the stream bottom
to live out their lives. |

Photo Source: Fisheries, Vol 19, No.
9, Sept 1993. |
Iowa
Species List and Conservation Status
| Scientific Name |
Common Name |
Current State Status |
| Actinonaias ligamentina |
Mucket |
None |
| Alasmidonta marginata |
Elktoe |
None |
| Alasmidonta viridis |
Slippershell |
Endangered |
| Amblema plicata |
Three Ridge |
None |
| Anondonta grandis |
Giant floater |
None |
| Anondonta imbecillis |
Paper pondshell |
None |
| Anodonta suborbiculata |
Flat floater |
None |
| Anodontoides ferussacianus |
Cylinder |
Threatened |
| Arcidens confragosus |
Rock-pocketbook |
None |
| Cumberlandia monodonta |
Spectacle case |
Endangered |
| Cyclonaias tuberculata |
Purple pimpleback |
Threatened |
| Ellipsaria lineolata |
Butterfly |
Threatened |
| Elliptio crassidens |
Elephant-ear |
None |
| Elliptio dilatata |
Spike |
None |
| Epioblasma triquetra |
Snuffbox |
None |
| Fusconaia ebena |
Ebonyshell |
None |
| Fusconaia flava |
Wabash pigtoe |
None |
| Lampsilis cardium |
Plain pocketbooket |
None |
| Lampsilis higginsi |
Higgins eye |
Endangered |
| Lampsilis siliquoidea |
Fatmucket |
None |
| Lampsilis teres |
Yellow sandshell |
Endangered |
| Lasmigona camplanata |
White heelsplitter |
None |
| Lasmigona compressa |
Creek heelsplitter |
Threatened |
| Lasmigona costata |
Fluted-shell |
Endangered |
| Leptodea fragilis |
Fragile papershell |
None |
| Leptodea leptodon |
Scaleshell |
Extirpated |
| Ligumia recta |
Black sandshell |
None |
| Ligumia subrostrata |
Common pondmussel |
None |
| Megalonaias nervosa |
Washboard |
None |
| Obliquaria reflexa |
Threehorn wartyback |
None |
| Obovaria olivaria |
Hickorynut |
None |
| Plethobasus cyphyus |
Sheepnose |
Endangered |
| Pleurobema coccineum |
Round pigtoe |
Endangered |
| Potamilus alatus |
Pink heelsplitter |
None |
| Potamilus capax |
Fat pocketbook |
Endangered |
| Potamilus ohiensis |
Pink papershell |
None |
| Quadrula fragosa |
Winged mapleleaf |
Endangered |
| Quadrula metanerva |
Monkeyface |
None |
| Quadrula nodulata |
Wartyback |
None |
| Quadrula pustulosa |
Pimpleback |
None |
| Quadrula quadrula |
Mapleleaf |
None |
| Simpsonaias ambigua |
Salamander mussel |
None |
| Strophitus undulatus |
Squawfoot |
Threatened |
| Toxolasma parvus |
Lilliput |
None |
| Tritogonia verrucosa |
Pistolgrip |
Endangered |
| Truncilla donaciformis |
Fawnsfoot |
None |
| Truncilla truncata |
Deertoe |
None |
| Venustaconcha ellipsiformis |
Ellipse |
Threatened |
Data
Collection Procedures
Examine Iowa Department of Transportation (IDOT) maps for
target streams and determine all practical access points.
IDOT maps are available by county by calling (515) 239-1282.
For large streams, space access areas no more than 5 miles
apart; for medium streams, no more than 3; and for small
streams, allow no more than 2 miles separation. Determine
the most efficient driving route. Clearly mark the sites
to be surveyed on the map.
Before collecting the data, record your name, the date, and
stream name on the data sheet. Recording the exact location
at which you are collecting data is essential. Provide a description
of the access area (e.g. Hwy. 30 bridge south of Ames, IA.)
on the data sheet. Document the site location by marking it
on the map.
If possible, take stream measurements from a data collection
point at least 100 meters (about a football field length)
from the access point to minimize its effect on stream characteristics
(EPA 1997). Indicate on the data sheet if the data is collected
upstream or downstream from the access point as well as the
distance.
While proceeding to the data collection point (100 meters
upstream or downstream from the access), complete a visual
survey of stream banks and shallow water for mussel shell
material and other aquatic animals (fish, crayfish and snails)
and plants (algae, duckweed, and pondweed). Complete the aquatic
plant and aquatic animal sections on the data sheet.
At the data collection point make the following measurements:
1. Stream width: Measure stream channel width in meters.
2. Water depth: Measure the depth (in cm) mid-channel
of the stream.
3. Stream Flow: Research indicates 0.3 m/sec is a
suitable stream velocity value for distinguishing between
fast and slow conditions (Plafkin et al. 1989). Calculate
the average velocity of the stream and indicate on the data
sheet whether the stream flow is fast or slow. Instructions
for measuring stream velocity are in Appendix
A.
4. Water Clarity: In shallow streams, water clarity
can be measured visually. If the streambottom is visible,
water clarity is high. In deeper streams, clarity can be measured
using a secchi disk, a round 20-cm diameter disk that is painted
in black and white quadrants. Water clarity is high when secchi
depth is greater than 60% of the water depth, moderate when
between 30-60% of the water depth and low when less than 30%
of the measured water depth. Instructions for using a secchi
disk are located in Appendix
A.
5. Substrate type: Evaluate substrate type (grab a
handful!) at 5 random places at the data collection point.
Indicate on the data sheet the dominant substrate type based
on the following categories:
Boulder (< 256 mm or 7")
Cobble (64-256 mm or 2"-7")
Gravel (2-64 mm or 0.05"-2")
Sand (0.06-2 mm - gritty)
Silt (0.004-0.06 mm)
Clay(<0.0004mm)
6. Bank vegetation: The absence or presence of bank
vegetation indicates the relative stability of a stream bank.
Streams banks with little or no vegetation are usually more
susceptible to bank erosion which adversely influences water
quality. Assess the bank vegetation present on the right and
left banks as absent or present.
7. Riparian zone: Land-use adjacent to the stream
influences stream habitat and water quality. Assess the land-use
and/or vegetation in the areas above the right and left banks.
Indicate the most dominant feature observed.
8. Additional Notes: Record miscellaneous observations
important to the assessing the habitat conditions of the stream
reach.
Appendix
A
Measuring Stream
Velocity
Measuring stream velocity is important because
it affects a variety of chemical, physical and biological
characteristics of a stream. The objective is to calculate
stream velocity by recording the amount of time (in seconds)
it takes a floating object to travel a known distance. A simple
way to do this is to attach a known length of line (2 meters
is sufficient) to a cork (see diagram below) and record the
time it takes the cork to float the distance of the full length
of the line in the stream current.

Field instructions:
1. Hold the cork and attached line at the surface of the
water. Position yourself so that you do not interfere with
stream flow.
2. Release the cork (hold on to the other end of the line!)
and start timing the float.
3. Stop timing when the cork has traveled the full distance
of the attached line.
4. Record the time it takes the line to fully extend in the
stream current.
5. Because velocity varies across the channel, repeat this
process 5 times, sending the float down different paths at
different location within the stream.
6. Calculate the average float time by dividing the sum of
the timed values by the number of float trials.
7. The average surface velocity (m/sec) of the stream is simply
the distance traveled (the length of the line) divided by
the average float time of the 5 float trials.
8. Because stream velocity varies from surface to bottom,
adjust this value to reflect the overall average velocity
of the stream, by multiplying the average velocity by the
correction factor 0.8 (Dunne and Leopold 1978).
Measuring Water Clarity
| Water clarity influences a variety of
stream characteristics. A measure of water clarity is
important as an indicator of many different processes
at work in the system. The simplest method of measuring
water clarity is a secchi disk. While a secchi disk
is typically used to measure water transparency in large,
deep water-bodies, it can be used to measure water clarity
in streams. The exception to this is in shallow, fast
running streams. A secchi disk is a 20cm disk with black
and white quadrants. For stream work, the disk is attached
to a wooden calibrated rod. The secchi depth measurements
described below are read from calibrated marks on the
rod.
Field instructions:
1. Lower the disk into the water until it disappears
from view and record this depth.
2. Drop the disc several cm more.
3. Slowly pull the disk back up and record the depth
at which it reappears.
4. Calculate the secchi depth by averaging these two
measured depths. |
 |
References
Dunn, T.B. and L.B. Leopold. 1978. Water in environmental
planning. W.H. Freeman, San Francisco, CA.
EPA. 1997. Revision to rapid bioassessment protocols for
use in streams and rivers: periphyton, benthic macroinvertebrates
and fish. EPA/841-D-97-002. U.S. EPA, Washington, D.C.
Frest, T.J. 1987. Mussel survey of selected interior Iowa
streams. University of Northern Iowa. Final Report to Iowa
Department of Natural Resources and U.S. Fish & Wildlife
Service. 354 pp.
Plafkin, J.L. M.T. Barbour, K.D. Porter, S.K. Gross and R.M.
Hughes. 1989. Rapid bioassessment protocols for use in streams
and rivers: benthic macroinvertebrates and fish. EPA/444/4-89-001.
U.S. EPA, Assess. and Watershed Protection Div., Washington,
D.C.
Project
Volunteers
The "Skunk River Navy"
This group of Iowa State University students evaluated
habitat conditions in the Skunk River, Story County. Lead
by Jim Colbert, participants attended a monitoring workshop
before heading to the field last Fall. In addition to collecting
valuable habitat information, they collected an unbelievable
amount of trash including a bath tub, hide-a-bed, and many,
many automobile tires!

The "Workshop"
September 1998 |
|

The "Field"
September 1998 |
The study sites are located on the Skunk River near Ames,
IA. Data were collected along a 500 m stream segment at
each site September 1998. A summary of the data recorded
is presented below.
| Skunk |
Site
1 (500 m) |
Site 2
(500 m) |
| Mean stream width |
17 m |
15 m |
| Average water depth (mid-channel) |
0.30 m |
0.34 m |
| Stream Flow |
N/A |
N/A |
| Water Clarity |
high |
high |
| Dominant substrate type |
sand |
gravel |
| Aquatic plants |
observed |
observed |
| Aquatic animals |
observed |
observed |
Iowa Central Community
College
Individuals from Iowa Central Community College are planning
to evaluated habitat conditions in streams located in
north central Iowa. The ICCC volunteers are providing
the project with a preliminary rapid-assessment of stream
quality. More pictures and data are forthcoming.

The "Field"
April 1999 |
|

The "Field"
April 1999 |

More
Detailed Mussel Information and Related Sites
For more information about freshwater mussels (i.e. anatomy
and life history, conservation, commercial harvest) and some
excellent pictures, check out these sites.
Mussels
of Illinois
Midwest
Field Guide to Freshwater Mussels
Mussels
of the World

|